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Recombinant MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody [MUC1/2980R]

Additional information

Catalog Number: 4582-RBM24
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4582-RBM24-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4582-RBM24-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4582-RBM24-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC1 Rabbit Recombinant Monoclonal Antibody (MUC1/2980R).

Recombinant MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody - Image 2

Western Blot Analysis of MCF-7 cell lysate using MUC1 Rabbit Recombinant Monoclonal Antibody (MUC1/2980R).

MUC1 Antibody in Western Blot (WB)

MUC1 Antibody in Western Blot (WB)

Western Blot Analysis of T47D cell lysate using MUC-1 Recombinant Rabbit Monoclonal Antibody (MUC1/2980R).

MUC1 Antibody in SDS-PAGE

MUC1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Mucin-1 Rabbit Monoclonal Antibody (MUC1/2980R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Flow
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot, Flow
Species Reactivity
Human
Clone
MUC1/2980R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Apical cell membrane, Cell membrane, Cytoplasm, Nucleus, Secreted
Gene Name
MUC1
Positive Control
Colon, endometrial carcinoma., MCF-7 or MDA-231 cells. Breast, Ovarian
Immunogen
Recombinant full-length human MUC1 protein
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Reactivity:

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Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Mouse Monoclonal Antibody (CDH16/1071).
7
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Additional information

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Reactivity:

, , , ,

Gene:

Host:

VIEW ALL ANTIBODIES

Other Information

Functions
  • The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack., The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4582
SwissProt
P15941
Chromosome Location
1q22
Mol. Weight of Antigen
122.1kDa
Alternate Names
Mucin-1, ADMCKD|ADMCKD1|ADTKD2|CA 15-3|CD227|Ca15-3|EMA|H23AG|KL-6|MAM6|MCD|MCKD|MCKD1|MUC-1|MUC-1/SEC|MUC-1/X|MUC1/ZD|PEM|PEMT|PUM

Summary

This MAb recognizes full-length MUC1 in a glycosylation-independent manner and can bind to the fully glycosylated protein.The dominant epitope of this MAb is APDTR in the VNTR region. It reacts with the core peptide of the MUC1 protein, which is a member of a family of mucin glycoproteins that are characterized by high carbohydrate content, O-linked oligosaccharides, high molecular weight (200kDa) and an amino acid composition rich in serine, threonine, proline and glycine. The core protein contains a domain of 20 amino-acid tandem repeats that functions as multiple epitopes for the MAb. Incomplete glycosylation of some tumor-associated mucins may lead to variable unmasking of the multiple peptide epitopes leading to the observed differences in staining intensity between normal and malignant tissues. This MAb reacts with both normal and malignant epithelia of various tissues including breast and colon.

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