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GFAP (Astrocyte & Neural Stem Cell Marker) Antibody [GFAP/9426]

Additional information

Catalog Number: 2670-MSM45
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
2670-MSM45-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
2670-MSM45-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2670-MSM45-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
GFAP Antibody in Western Blot (WB)

GFAP Antibody in Western Blot (WB)

Western Blot Analysis of human brain tissue lysate using GFAP Mouse Monoclonal Antibody (GFAP/9426).

GFAP Antibody in SDS-PAGE

GFAP Antibody in SDS-PAGE

SDS-PAGE Analysis Purified GFAP Mouse Monoclonal Antibody (GFAP/9426). Confirmation of Purity and Integrity of Antibody.

GFAP Antibody in Immunohistochemistry (IHC (P))

GFAP Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human brain stained with GFAP Mouse Monoclonal Antibody (GFAP/9426). Inset: PBS instead of primary antibody; secondary only negative control.

GFAP Antibody in Western Blot (WB)

GFAP Antibody in Western Blot (WB)

Western blot analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain and Guinea pig Brain tissue lysates using GFAP Mouse Monoclonal Antibody (GFAP/9426).

GFAP Antibody in Western Blot (WB)

GFAP Antibody in Western Blot (WB)

Western Blot Analysis of Human Brain, Human Heart, Human Intestine, Human Kidney, Human Liver, Human Lung, Human Ovary, Human Pancreas, Human Prostrate, Human Skeletal Muscle, Human Skin, Human Spleen, Human Stomach and Human Testis tissue lysates using GFAP Recombinant Rabbit Monoclonal Antibody (GFAP/9426).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
GFAP/9426
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cytoplasm
Gene Name
GFAP
Positive Control
Human brain.
Immunogen
Recombinant fragment (around aa150-350) of human GFAP protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Key References
  • McLendon, R.E. and Bigner, D.D. 1994. Immunohistochemistry of the glial fibrillary acidic protein: basic and applied considerations. Brain Pathol. 4: 221-228.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2670
SwissProt
P14136
Chromosome Location
17q21.31
Mol. Weight of Antigen
~50kDa
Alternate Names
Glial fibrillary acidic protein, Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

Summary

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

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