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CD202b / TIE2 / TEK (Tyrosine Kinase with Ig and EGF Homology Domains 2) Antibody [TEK/9411]

In Stock
Catalog Number Formulation Size Price
7010-MSM10-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
7010-MSM10-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
7010-MSM10-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
TEK / TIE2 Antibody in Immunohistochemistry (IHC (P))

TEK / TIE2 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human testis stained with TEK Mouse Monoclonal Antibody (TEK/9411). Inset: PBS instead of primary antibody; secondary only negative control.

TEK Antibody in SDS-PAGE

TEK Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified TEK Mouse Monoclonal Antibody (TEK/9411). Confirmation of Purity and Integrity of Antibody.

TEK / TIE2 Antibody in Immunohistochemistry (IHC (P))

TEK / TIE2 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with TEK Mouse Monoclonal Antibody (TEK/9411). Inset: PBS instead of primary antibody; secondary only negative control.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Immuno Oncology
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane, Cytoplasm, Secreted
Gene Name
TEK
Positive Control
Human lung, placenta or spleen.
Immunogen
Recombinant fragment (around aa700-1000) of human TEK protein (exact sequence is proprietary)
Alternate Names
TEK, Angiopoietin 1 receptor antibody; Angiopoietin-1 receptor antibody; CD202b antibody; CD202b antigen antibody; Endothelial tyrosine kinase antibody; Endothelium specific receptor tyrosine kinase 2 antibody; hTIE 2 antibody; hTIE2 antibody; Hyk antibody; p140 TEK antibody; Soluble TIE2 variant 1 antibody; Soluble TIE2 variant 2 antibody; Tek antibody; tek tyrosine kinase antibody; TEK tyrosine kinase endothelial antibody; tek tyrosine kinase, endothelial antibody; TIE 2 antibody; TIE2 antibody; TIE2_HUMAN antibody; Tunica interna endothelial cell kinase antibody; Tyrosine kinase with Ig and EGF homology domains 2 antibody; Tyrosine kinase with Ig and EGF homology domains-2 antibody; Tyrosine protein kinase receptor TEK antibody ;Tyrosine protein kinase receptor TIE 2 antibody; Tyrosine-protein kinase receptor TEK antibody; Tyrosine-protein kinase receptor TIE-2 antibody; Venous malformations multiple cutaneous and mucosal antibody; VMCM 1 antibody VMCM antibody; VMCM1 antibody, TIE2; VMCM; GLC3E; TIE-2; VMCM1; CD202B

Database Links

Entrez Gene ID
7010
SwissProt
Q02763

Additional Information

Clone
TEK/9411
Chromosome Location
9p21.2
Mol. Weight of Antigen
65kDa

Related Antibodies

View All TEK Antibodies

Functions

  • Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1; SHC1 and TIE1.

Key References

  • Pan, Xu et al. “Tumour vasculature at single-cell resolution.” Nature vol. 632,8024 (2024): 429-436. doi:10.1038/s41586-024-07698-1
  • Akwii, Racheal G et al. “Role of Angiopoietin-2 in Vascular Physiology and Pathophysiology.” Cells vol. 8,5 471. 17 May. 2019, doi:10.3390/cells8050471
  • Karabid, Ninelia Minaskan et al. “Angpt2/Tie2 autostimulatory loop controls tumorigenesis.” EMBO molecular medicine vol. 14,5 (2022): e14364. doi:10.15252/emmm.202114364

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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