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Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody [BRD469]

In Stock
Catalog Number Formulation Size Price
MSM1-469-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
MSM1-469-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
MSM1-469-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Bromodeoxyuridine Antibody in Immunohistochemistry (IHC (P))

Bromodeoxyuridine Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with BrdU Mouse Monoclonal Antibody (BRD469).

Bromodeoxyuridine Antibody in Immunofluorescence (IF)

Bromodeoxyuridine Antibody in Immunofluorescence (IF)

Formalin-fixed, paraffin-embedded Rat Kidney stained with CF488-conjugated BrdU Mouse Monoclonal Antibody (BRD469).

Bromodeoxyuridine Antibody in SDS-PAGE

Bromodeoxyuridine Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified BrdU Mouse Monoclonal Antibody (BRD469). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
All species
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus
Gene Name
N/A
Positive Control
Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.
Immunogen
Bromodeoxyuridine (BrdU) conjugated to KLH
Alternate Names
N/A, N/A, Bromodeoxyuridine, BUdr

Database Links

Entrez Gene ID
Not Applicable
SwissProt
N/A

Additional Information

Clone
BRD469
Mol. Weight of Antigen
307Da

Related Antibodies

View All N/A Antibodies

Functions

  • N/A

Key References

  • Welberg JW et. al. Journal of Clinical Pathology, 1990, 43(6):453-6.
  • Williams LS et. al. Cytometry, 1990, 11(4):490-7.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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