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Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody [BRD469]

Additional information

Catalog Number: MSM1-469
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
MSM1-469-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
MSM1-469-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
MSM1-469-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Bromodeoxyuridine Antibody in Immunohistochemistry (IHC (P))

Bromodeoxyuridine Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded Mouse Small Intestine stained with BrdU Mouse Monoclonal Antibody (BRD469).

Bromodeoxyuridine Antibody in Immunofluorescence (IF)

Bromodeoxyuridine Antibody in Immunofluorescence (IF)

Formalin-fixed, paraffin-embedded Rat Kidney stained with CF488-conjugated BrdU Mouse Monoclonal Antibody (BRD469).

Bromodeoxyuridine Antibody in SDS-PAGE

Bromodeoxyuridine Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified BrdU Mouse Monoclonal Antibody (BRD469). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
All species
Clone
BRD469
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
N/A
Positive Control
Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.
Immunogen
Bromodeoxyuridine (BrdU) conjugated to KLH
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • N/A
Key References
  • Welberg JW et. al. Journal of Clinical Pathology, 1990, 43(6):453-6.
  • Williams LS et. al. Cytometry, 1990, 11(4):490-7.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
Not Applicable
SwissProt
N/A
Mol. Weight of Antigen
307Da
Alternate Names
N/A, N/A, Bromodeoxyuridine, BUdr

Summary

It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

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