Wilm’s Tumor 1 (WT1) (Wilm’s Tumor & Mesothelial Marker) Antibody [WT1/7714]

Name: Wilm's Tumor 1 (WT1) (Wilm's Tumor &amp; Mesothelial Marker) Antibody
SKU: 7490-MSM14
Availability: InStock

In Stock

Flat Rate Domestic: $95 | Orders outside the US - for Order Information | Ships next business day

Applications & Dilutions

Immunohistochemistry (IHC)

1-2ug/ml

IHC Protocol

30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5′-GCG(T/G)GGGCG-3′ (PubMed:17716689, PubMed:25258363, PubMed:7862533). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Immunohistochemistry

Species Reactivity

Human

Isotype / Light Chain

IgG1 / Kappa

Cellular Localization

Cytoplasm, Nucleolus, Nucleoplasm, Nucleus, Nucleus speckle

Gene Name

WT1

Positive Control

Expressed in the kidney and a subset of hematopoietic cells

Immunogen

Recombinant human WT1 protein

Alternate Names

Wilms tumor protein, AWT1|GUD|NPHS4|WAGR|WIT-2|WT-1|WT33

Database Links

Entrez Gene ID

7490

SwissProt

P19544

Additional Information

Clone

WT1/7714

Chromosome Location

11p13

Mol. Weight of Antigen

49.19kDa

Purification

Protein G

Functions

Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:17716689, PubMed:25258363, PubMed:7862533). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Wilm’s Tumor 1 (WT1) (Wilm’s Tumor & Mesothelial Marker) Antibody [WT1/7714]

Applications & Dilutions

Summary

Product Properties & Targets

Database Links

Additional Information

Related Antibodies

Wilm’s Tumor 1 (WT1) (Wilm’s Tumor & Mesothelial Marker) Antibody [6F-H2]

Wilm’s Tumor 1 (WT1) (Wilm’s Tumor & Mesothelial Marker) Antibody [SPM361]

Wilm’s Tumor 1 (WT1) (Wilm’s Tumor & Mesothelial Marker) Antibody [WT1/857]

Wilm’s Tumor 1 (WT1) (Wilm’s Tumor & Mesothelial Marker) Antibody [WT1/857 + 6F-H2]