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TCF7 Antibody [TCF7/7631]

Additional information

Catalog Number: 6932-MSM1
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6932-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6932-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6932-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
TCF7 Antibody

SDS-PAGE Analysis of Purified TCF7 Mouse Monoclonal Antibody (TCF7/7631). Confirmation of Purity and Integrity of Antibody.

TCF7 Antibody - Image 2

Formalin-fixed, paraffin-embedded human lymph node stained with TCF7 Mouse Monoclonal Antibody (TCF7/7631). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

TCF7 Antibody - Image 3

Formalin-fixed, paraffin-embedded human tonsil stained with TCF7 Mouse Monoclonal Antibody (TCF7/7631). Inset: PBS instead of primary antibody; secondary only negative control.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
TCF7/7631
Isotype
IgG2
Light Chain
Kappa
Cellular Localization
Membrane.
Gene Name
TCF7
Positive Control
adrenal gland or colon.|Human spleen|lung
Immunogen
Recombinant fragment (around aa200-400) of human TCF7 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. Represses expression of the T-cell receptor gamma gene in alpha-beta T-cell lineages (By similarity). Required for the development of natural killer receptor-positive lymphoid tissue inducer T-cells (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes.
Key References
  • Van de Wetering, M., et al. 1992. The human T cell transcription factor-1 gene. Structure, localization, and promoter characterization. J. Biol. Chem. 267: 8530-8536.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6932
SwissProt
P36402
Chromosome Location
5q31.1
Mol. Weight of Antigen
41.6kDa
Alternate Names
Transcription factor 7, T-cell-specific transcription factor 1, Transcription Factor 7 (T-Cell Specific, HMG-Box); T-Cell-Specific Transcription Factor 1; T-Cell Factor 7

Summary

Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. Represses expression of the T-cell receptor gamma gene in alpha-beta T-cell lineages (By similarity). Required for the development of natural killer receptor-positive lymphoid tissue inducer T-cells (By similarity). TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes.

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