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SERPINB5 / Maspin Antibody [SERPINB5/4977]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
5268-MSM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
5268-MSM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
5268-MSM7-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

Maspin is structurally a serine protease inhibitor (serpin) that was initially isolated from normal human mammary epithelial cells. Serpins are a family of proteins that inhibit Chymotrypsin-like serine proteinases. Serpins control activated proteinases and several are involved in the regulation of cell death. Maspin is found in the extracellular matrix and at the plasma membrane. Maspin has been shown to act at the cell surface to block cell motility and inhibit invasion of breast and prostate cancer cells. Maspin is present in normal mammary epithelial cells but is absent in many tumor cell lines, yet no major structural alterations of the Maspin gene have been identified in tumor cells. Similarly, Maspin is expressed in normal prostate cells and downregulated or absent in prostate tumor cells.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Research Areas
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Extracellular space, Secreted
Gene Name
Positive Control
Squamous epithelium or gastrointestinal tissue.
Immunogen
Recombinant fragment of human SERPINB5 protein (around aa1-200) (exact sequence is proprietary)
Alternate Names
Serpin B5, Maspin, Peptidase inhibitor 5, Maspin; Ovalbumin; PI-5; PI5; protease inhibitor 5 (maspin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5; Serpin B5; serpin family B member 5; Serpin peptidase inhibitor, clade B (ovalbumin), member 5; Spi5; Spi7

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
SERPINB5/4977
Chromosome Location
18q21.33
Mol. Weight of Antigen
42kDa

Functions

  • Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.

Key References

  • Zou, Z., et al. 1994. Maspin, a serpin with tumor-suppressing activity in human mammary epithelial cells. Science 263: 526-529.
  • Zheng HC, et al. Cytoplasmic and nuclear maspin expression in lung carcinomas: an immunohistochemical study using tissue microarrays. Appl Immunohistochem Mol Morphol. 2008;16(5):459-465.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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