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Recombinant S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody [S100A4/2750R]

Additional information

Catalog Number: 6275-RBM4
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6275-RBM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6275-RBM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6275-RBM4-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody

Formalin-fixed, paraffin-embedded human Placenta stained with S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R).

Recombinant S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody - Image 2

Immunofluorescence staining of A549 cells using S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R) followed by goat anti-Mouse IgG-CF488 (green). Nuclei are stained with Reddot.

Recombinant S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody - Image 3

Flow Cytometric Analysis of A549 cells using S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

Recombinant S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody - Image 4

SDS-PAGE Analysis Purified S100A4Recombinant Rabbit Monoclonal Antibody (S100A4/2750R). Confirmation of Purity and Integrity of Antibody.

S100A4 Antibody in Western Blot (WB)

S100A4 Antibody in Western Blot (WB)

Western Blot Analysis of Human Heart, Human Lung, Human Spleen, Human Ovary, A549, and HeLa lysates using S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R).

S100A4 Antibody in Western Blot (WB)

S100A4 Antibody in Western Blot (WB)

Western Blot Analysis of Heart, Lung and Spleen tissue lysates of different species using S100A4 Recombinant Rabbit Monoclonal Antibody (S100A4/2750R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
S100A4/2750R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm, Nucleus, Secreted
Gene Name
S100A4
Positive Control
HeLa, A549 or A375 cells. Heart, Lung, Spleen, Placenta.
Immunogen
Recombinant fragment (around aa2-101) of the human S100A4 protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Calcium-binding protein that plays a role in various cellular processes including motility, angiogenesis, cell differentiation, apoptosis, and autophagy (PubMed:16707441, PubMed:23752197, PubMed:30713770). Increases cell motility and invasiveness by interacting with non-muscle myosin heavy chain (NMMHC) IIA/MYH9 (PubMed:16707441). Mechanistically, promotes filament depolymerization and increases the amount of soluble myosin-IIA, resulting in the formation of stable protrusions facilitating chemotaxis (By similarity). Also modulates the pro-apoptotic function of TP53 by binding to its C-terminal transactivation domain within the nucleus and reducing its protein levels (PubMed:23752197). Within the extracellular space, stimulates cytokine production including granulocyte colony-stimulating factor and CCL24 from T-lymphocytes (By similarity). In addition, stimulates T-lymphocyte chemotaxis by acting as a chemoattractant complex with PGLYRP1 that promotes lymphocyte migration via CCR5 and CXCR3 receptors (PubMed:26654597, PubMed:30713770).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6275
SwissProt
P26447
Chromosome Location
1q21.3
Mol. Weight of Antigen
11.73kDa
Alternate Names
Protein S100-A4, 18A2|42A|CAPL|FSP1|MTS1|P9KA|PEL98

Summary

S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, 64 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

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