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Formalin-fixed, paraffin-embedded human melanoma stained with NGFR Rabbit Recombinant Monoclonal Antibody (NGFR/1997R). Inset: PBS instead of primary antibody; secondary only negative control.
Formalin-fixed, paraffin-embedded human pancreas stained with NGFR Rabbit Recombinant Monoclonal Antibody (NGFR/1997R).
Formalin-fixed, paraffin-embedded human melanoma stained with NGFR Rabbit Recombinant Monoclonal Antibody (NGFR/1997R). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.
SDS-PAGE Analysis Purified NGFR Rabbit Recombinant Monoclonal Antibody (NGFR/1997R). Confirmation of Purity and Integrity of Antibody.
Analysis of Protein Array containing more than 19,000 full-length human proteins using NGFR-Monospecific Rabbit Recombinant Monoclonal Antibody (NGFR/1997R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western Blot Analysis of Human Brain tissue lysate using NGFR Recombinant Rabbit Monoclonal Antibody (NGFR/1997R).
Western Blot Analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain, Guinea pig Brain, and Rabbit Brain tissue lysates using Nerve Growth Factor Receptor Recombinant Rabbit Monoclonal Antibody (NGFR/1997R).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
0
3
3
3
6
5
It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.
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