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Western Blot Analysis of Ramos and Raji cell lysates using MCM5 Recombinant Rabbit Monoclonal Antibody (MCM5/13320R).
SDS-PAGE Analysis of Purified MCM5 Recombinant Rabbit Monoclonal Antibody (MCM5/13320R). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded human tonsil stained with MCM5 Recombinant Rabbit Monoclonal Antibody (MCM5/13320R). Inset: PBS instead of primary antibody; secondary only negative control.
Western Blot validation of anti-MCM5 antibody. MCM5 protein expression was compared in wild-type (WT) and MCM5 shRNA Knockdown (KD) HeLa cells (30ug of total protein). Loading control is shown below.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double- stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation of the MCM complex (composed of at least MCM proteins 2-6) during apoptosis. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity; and MCM5 is involved in IFN-γ-induced Stat1α transcription activation.
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