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Recombinant CD10 (Membrane Metalloendopeptidase) Antibody [56C6]

Additional information

Catalog Number: 4311-MSM54
Gene:

Host:

Reactivity:

, , , , ,

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4311-MSM54-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4311-MSM54-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4311-MSM54-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD10 Antibody in Western Blot (WB)

CD10 Antibody in Western Blot (WB)

Western Blot Analysis of human kidney lysate using CD10 Mouse Monoclonal Antibody (56C6).

CD10 Antibody in Western Blot (WB)

CD10 Antibody in Western Blot (WB)

Western Blot Analysis of Human Kidney, Mouse Kidney, Rat Kidney, Hamster Kidney, Guinea pig Kidney and Rabbit Kidney tissue lysates using CD10 Mouse Monoclonal Antibody (56C6).

CD10 Antibody in SDS-PAGE

CD10 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD10 Recombinant Mouse Monoclonal Antibody (56C6). Confirmation of Purity and Integrity of Antibody.

CD10 Antibody in Immunohistochemistry (IHC (P))

CD10 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human kidney stained with CD10 Mouse Monoclonal Antibody (56C6). Inset: PBS instead of primary antibody; secondary only negative control.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Guinea Pig, Hamster, Human, Mouse, Rabbit, Rat
Clone
56C6
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell membrane
Gene Name
MME
Positive Control
Tonsil, small intestine or kidney.
Immunogen
Recombinant full-length human CD10 protein
Expression System
Hybridoma
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids (PubMed:15283675, PubMed:6208535, PubMed:6349683, PubMed:8168535). Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond (PubMed:17101991, PubMed:6349683). Catalyzes cleavage of bradykinin, substance P and neurotensin peptides (PubMed:6208535). Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9 (PubMed:15283675, PubMed:6349683). Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)) (PubMed:16254193, PubMed:2531377, PubMed:2972276). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4311
SwissProt
P08473
Chromosome Location
3q25.2
Mol. Weight of Antigen
85.51kDa
Alternate Names
Neprilysin, CALLA|CD10|CMT2T|NEP|SCA43|SFE

Summary

Recognizes a 100kDa glycoprotein, identified as CD10, also known as Common Acute Lymphocytic Leukemia Antigen (CALLA). It is a cell surface enzyme with neutral metalloendopeptidase activity, which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue.CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

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