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Recombinant Beta-Parvin Antibody [PARVB/8214R]

Additional information

Catalog Number: 29780-RBM3
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
29780-RBM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
29780-RBM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
29780-RBM3-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Beta-Parvin Antibody

Formalin-fixed, paraffin-embedded human stomach stained with Beta-Parvin Recombinant Rabbit Monoclonal Antibody (PARVB/8214R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Recombinant Beta-Parvin Antibody - Image 2

SDS-PAGE Analysis of Purified Parvin Recombinant Rabbit Monoclonal Antibody (PARVB/8214R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
PARVB/8214R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Focal adhesion, Lamellipodium, Myofibril, Sarcomere, Z line
Gene Name
PARVB
Positive Control
HeLa cell lysate. HeLa cells. Human stomach.
Immunogen
Recombinant fragment (around aa1-91) of the human Parvin, beta (PARVB) protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Adapter protein that plays a role in integrin signaling via ILK and in activation of the GTPases CDC42 and RAC1 by guanine exchange factors, such as ARHGEF6. Is involved in the reorganization of the actin cytoskeleton and formation of lamellipodia. Plays a role in cell adhesion, cell spreading, establishment or maintenance of cell polarity, and cell migration.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
29780
SwissProt
Q9HBI1
Chromosome Location
22q13.31
Mol. Weight of Antigen
41.71kDa
Alternate Names
Beta-parvin, CGI-56

Summary

Beta-parvin, also known as affixin, is a focal adhesion protein belonging to a family of ILKbinding proteins that contain tandem calponin homolog domains. Along with Alpha-parvin, Beta-parvin is one of the most widely researched and characterized members of this family. These two proteins interact with the ILK kinase domain in a way that is mutually exclusive and regulates both the activity of the kinase and actin cytoskeleton adhesion. Studies have shown Beta-parvin to be downregulated within human breast cancer cells, and that its expression may influence the progression of breast cancer (Johnstone, 2008). Additional research indicates a critical role for Beta-parvin in the regulation of cardiac contractility when it is in complex with ILK (Hannigan, 2007).

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