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Recombinant NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) Antibody [ENO/8253R]

Additional information

Catalog Number: 2026-RBM18
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
2026-RBM18-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2026-RBM18-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2026-RBM18-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) Antibody

Formalin-fixed, paraffin-embedded human pancreas stained with NSE gamma Recombinant Rabbit Monoclonal Antibody (ENO/8253R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified NSE gamma Recombinant Rabbit Monoclonal Antibody (ENO/8253R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
ENO/8253R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm.
Gene Name
ENO2
Positive Control
cerebellum or pheochromocytoma.|Jurkat cells. Human pancreas
Immunogen
Recombinant fragment of human NSE gamma (around aa234-434) (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
Key References
  • Verma, M., et al. 1994. DNA sequences encoding enolase are remarkably conserved from yeast to mammals. Life Sci. 55: 893-899.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2026
SwissProt
P09104 Human
Chromosome Location
12p13
Mol. Weight of Antigen
~50kDa
Alternate Names
Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, 2-phospho-D-glycerate hydrolyase; ENO2; ENOG; Enolase 2 gamma neuronal; Enolase2; Gamma-enolase; Neural enolase; Neuron specific gamma enolase; Neuron-specific enolase; NSE

Summary

This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. �It it be usually employed in combination with other markers such as Synaptophysin, �Chromogranin A, and Neurofilament. �

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