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MAP2 Antibody [MAP2/7673]

Additional information

Catalog Number: 4133-MSM1
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4133-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4133-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4133-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
MAP2 Antibody

Formalin-fixed, paraffin-embedded human brain stained with MAP2 Mouse Monoclonal Antibody (MAP2/7673). Inset: PBS instead of primary antibody; secondary only negative control.

MAP2 Antibody - Image 2

Formalin-fixed, paraffin-embedded human brain stained with MAP2 Mouse Monoclonal Antibody (MAP2/7673). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

MAP2 Antibody - Image 3

SDS-PAGE Analysis of Purified MAP2 Mouse Monoclonal Antibody (MAP2/7673). Confirmation of Purity and Integrity of Antibody.

  • ELISA
  • IF
  • IHC
  • WB
  • Immunocytochemistry
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry
Species Reactivity
Human
Clone
MAP2/7673
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cytoplasm. Cytoskeleton.
Gene Name
MAP2
Positive Control
Human brain.
Immunogen
Recombinant fragment (around aa1500-1700) of human MAP2 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Key References
  • Neal, A.P., et al. 2010. Lead exposure during synaptogenesis alters vesicular proteins and impairs vesicular release: potential role of NMDA receptor-dependent BDNF signaling. Toxicol. Sci. 116: 249-263.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4133
SwissProt
P11137Human
Chromosome Location
2q34
Mol. Weight of Antigen
280/70kDa
Alternate Names
Microtubule-associated protein 2, DKFZp686I2148; MAP dendrite specific; MAP2; MAP2A; MAP2B; MAP2C; Microtubule-associated protein 2;

Summary

Microtubules, the primary component of the cytoskeletal network, interact with proteins called microtubule-associated proteins (MAPs). The microtubuleassociated proteins can be divided into two groups, structural and dynamic. The structural microtubule-associated proteins, MAP-1A, MAP-1B, MAP-2A, MAP-2B and MAP-2C, stimulate tubulin assembly, enhance microtubule stability and influence the spatial distribution of microtubules within cells. Both MAP-1 and, to a greater extent, MAP-2 have been implicated as agents of microtubule depolymerization by suppressing the dynamic instability of the microtubules. The suppression of microtubule dynamic instability by the MAP proteins is thought to be associated with phosphorylation of the MAPs.

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