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CD29 (Stem Cell Marker) Antibody [ITGB1/3613]

Additional information

Catalog Number: 3688-MSM3
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3688-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3688-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3688-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD29 (Stem Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human Lung stained with CD29 Mouse Monoclonal Antibody (ITGB1/3613).

CD29 (Stem Cell Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human kidney stained with CD29 Mouse Monoclonal Antibody (ITGB1/3613).

CD29 (Stem Cell Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified CD29 Mouse Monoclonal Antibody (ITGB1/3613). Confirmation of Purity and Integrity of Antibody.

CD29 Antibody in Western Blot (WB)

CD29 Antibody in Western Blot (WB)

Western Blot Analysis of Human Kidney tissue lysate using CD29 Mouse Monoclonal Antibody (ITGB1/3613).

  • ELISA
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
ITGB1/3613
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell junction, Cell membrane, Cell projection, Cell surface, Cleavage furrow, Focal adhesion, Invadopodium membrane, Lamellipodium, Melanosome, Recycling endosome, Ruffle, Ruffle membrane, Sarcolemma
Gene Name
ITGB1
Positive Control
HT1080 whole cell lysate. HeLa cell line. Human Kidney.
Immunogen
Purified human beta 1 integrin from HT1080 fibrosarcoma cell extract
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion (By similarity). Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. When associated with alpha-7 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). ITGA5:ITGB1 is a receptor for IL1B and binding is essential for IL1B signaling (PubMed:29030430). ITGA5:ITGB3 is a receptor for soluble CD40LG and is required for CD40/CD40LG signaling (PubMed:31331973). Plays an important role in myoblast differentiation and fusion during skeletal myogenesis (By similarity).
  • Interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro).
  • Isoform 5 displaces isoform 1 in striated muscles.
  • (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8.
  • (Microbial infection) Acts as a receptor for Cytomegalovirus/HHV-5.
  • (Microbial infection) Acts as a receptor for Epstein-Barr virus/HHV-4.
  • (Microbial infection) Integrin ITGA5:ITGB1 acts as a receptor for Human parvovirus B19.
  • (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus.
  • (Microbial infection) Acts as a receptor for Mammalian reovirus.
  • (Microbial infection) In case of HIV-1 infection, integrin ITGA5:ITGB1 binding to extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • (Microbial infection) Interacts with CotH proteins expressed by fungi of the order mucorales, the causative agent of mucormycosis, which plays an important role in epithelial cell invasion by the fungi (PubMed:32487760). Integrin ITGA3:ITGB1 may act as a receptor for R.delemar CotH7 in alveolar epithelial cells, which may be an early step in pulmonary mucormycosis disease progression (PubMed:32487760).
  • (Microbial infection) May serve as a receptor for adhesin A (nadA) of N.meningitidis.
Key References
  • Tamkun, J.W. et al. Cell 46: 271-282 (1986)
  • Loftus, J.C. et al. Science 249: 915-918 (1990)
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3688
SwissProt
P05556
Chromosome Location
10p11.2
Mol. Weight of Antigen
110kDa (non-reduced) & 130kDa (reduced)
Alternate Names
Integrin beta-1, Fibronectin receptor subunit beta, Glycoprotein IIa, VLA-4 subunit beta, Fibrinogen Receptor beta subunit; FNRB; Glycoprotein IIa (GPIIA); Integrin beta-1 (ITGB1); integrin VLA-4 beta subunit; MDF2; MSK12; Very Late Activation Protein beta Polypeptide; VLA-4 subunit beta; VLA-BETA; VLAB

Summary

Recognizes CD29. Binds to the cell membrane of T- and B- lymphocytes, monocytes, platelets, fibroblasts, endothelial cells, mast cells and smooth muscle cells. CD29 is an adhesion receptor and co-stimulatory molecule. This antigen was initially characterized as GPIIa on platelets and as the common b1 subunit of the very late antigen (VLA) protein family. CD29 forms a non-covalent heterodimeric complex with integrin alpha subunits. It also mediates adhesion to invasion and thrombospondin.

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