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Home >> Antibodies >> GAD1 / GAD67 (GABAergic Neuronal Marker)

GAD1 / GAD67 (GABAergic Neuronal Marker)

Mouse Monoclonal Antibody [Clone GAD1/2391]

Catalog #

Size

20 ug
100 ug
100 ug

Price (USD)

199.00
429.00
429.00

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Catalog No

Size

20 ug
100 ug
100 ug

Formulation

Purified Ab with BSA and Azide at 200ug/ml
Purified Ab with BSA and Azide at 200ug/ml
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Price (USD)

199.00
429.00
429.00
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Formalin-fixed, paraffin-embedded human Brain stained with GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2391).

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SDS-PAGE Analysis Purified GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2391). Confirmation of Integrity and Purity of Antibody.

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Western Blot Analysis of human Brain tissue lysate using GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2391).

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Analysis of Protein Array containing more than 19,000 full-length human proteins using GAD1 (GAD67) Mouse Monoclonal Antibody (GAD1/2391)

Product Details

Synonyms

67kDa glutamic acid decarboxylase; CPSQ1; DCE1; GAD67; GAD1; Glutamate decarboxylase 1; SCP

Positive Control

K-562 or HEK293 cells. Pancreas.

Known Applications & Suggested Dilutions

ELISA, Western Blot, IHC-P         More Details

ELISA (For coating, order antibody without BSA)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunogen

Recombinant human GAD1 (GAD67) protein fragment (around aa 72-135) (exact sequence is proprietary)

Cellular Localization

Cytoplasmic

Species Reactivity

Human.

Host / Ig Isotype

Mouse / IgG1, kappa

Mol. Weight of Antigen

67kDa

Specificity & Comments

This MAb recognizes a protein of 67kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1. GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.

Related Products



200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Key References

  1. Kaufman DL, Erlander MG, Clare-Salzler M, Atkinson MA, Maclaren NK, Tobin AJ. Autoimmunity to two forms of glutamate decarboxylase in insulin-dependent diabetes mellitus. J Clin Invest. 1992 Jan;89(1):283-92.

Bioinformatics

  •  Entrez Gene ID: 2571
  •  Gene Symbol: GAD1
  •  SwissProt: Q99259
  •  Chromosome Location: 2q31.1
  •  Unigene: 420036
NBT02391