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Cytokeratin, Basic (Type II or HMW) Antibody [DE-SQ]

In Stock
Catalog Number Formulation Size Price
MSM29-860-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
MSM29-860-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
MSM29-860-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Cytokeratin, Basic (Type II or HMW) Antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin, HMW Mouse Monoclonal Antibody (DE-SQ).

KRT76 Antibody in SDS-PAGE

KRT76 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Keratin, type II cytoskeletal 2 oral Mouse Monoclonal Antibody (DE-SQ). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

This antibody is specific to cytokeratin 13, 14, 15 and 16. This antibody stains squamous carcinomas and adeno-squamous carcinomas but does not stain adenocarcinoma. This antibody is useful in differential staining of squamous carcinomas from adenocarcinomas and to differentiate benign and malignant tumors of prostate glands.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Developmental Biology
Isotype / Light Chain
IgG1 / Kappa
Gene Name
KRT76
Positive Control
Ectocervix or Squamous carcinoma., Esophagus, PC12 cells. Tonsil, prostate, Skin
Immunogen
Cytoskeletal fraction prepared from human gingiva by detergent/salt extraction
Alternate Names
Keratin, type II cytoskeletal 2 oral, Cytokeratin-2P, Keratin-76, Type-II keratin Kb9, KRT2B; KRT2P; HUMCYT2A; Keratin, type II Cytoskeletal 2 oral; K76; Keratin 2p (K2P); Keratin-76; Cytokeratin-2P (CK-2P; Type-II Keratin Kb9

Database Links

Entrez Gene ID
51350
SwissProt
Q01546

Additional Information

Clone
DE-SQ
Chromosome Location
12q13.13
Mol. Weight of Antigen
50-67kDa

Related Antibodies

View All KRT76 Antibodies

Functions

  • Probably contributes to terminal cornification.

Key References

  • Moinfar F et. al. Am J Surg Pathol 1999;23(9):1048-58
  • Varma M et. al. Mod Pathol 1999;12(5):472-8

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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