Wilm’s Tumor 1 (WT1) (Wilm’s Tumor & Mesothelial Marker) Antibody []

Name: Wilm&#8217;s Tumor 1 (WT1) (Wilm&#8217;s Tumor &amp; Mesothelial Marker) Antibody
SKU: 7490-RBP5
Availability: InStock

In Stock

Flat Rate Domestic: $60 | Orders outside the US - for Order Information | Ships next business day

Formalin-fixed, paraffin-embedded human Mesothelioma stained with Wilm's Tumor Rabbit Polyclonal Antibody Antibody.

Applications & Dilutions

Immunohistochemistry (IHC)

1-2ug/ml

IHC Protocol

30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Product Properties & Targets

Antibody Type

Rabbit Polyclonals

Host

Rabbit

Applications

Immunohistochemistry

Species Reactivity

Human, Rat

Research Areas

Cancer, Developmental Biology, Cardiac Stem Cells, Stem Cell Differentiation

Isotype / Light Chain

IgG / Kappa

Cellular Localization

Cytoplasm, Nucleolus, Nucleoplasm, Nucleus, Nucleus speckle

Gene Name

WT1

Positive Control

K562 cells. Wilm's Tumor, mesothelioma or fetal kidney.

Immunogen

Recombinant human WT1 protein

Alternate Names

Wilms tumor protein, WT33, WT1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1

Database Links

Entrez Gene ID

7490

SwissProt

P19544

Additional Information

Chromosome Location

11p13

Mol. Weight of Antigen

47-55kDa

Functions

Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:7862533, PubMed:17716689, PubMed:25258363). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).

Key References

Rauscher FJ. The WT1 Wilms tumor gene product: A developmentally regulated transcription factor in the kidney that functions as a tumor suppressor. FASEB J 1993; 7:896

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Wilm’s Tumor 1 (WT1) (Wilm’s Tumor & Mesothelial Marker) Antibody”