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TCF4 (Transcription Factor 4) Antibody [TCF4/2252]

Additional information

Catalog Number: 6925-MSM2
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6925-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6925-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6925-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
TCF4 (Transcription Factor 4) Antibody

Formalin-fixed, paraffin-embedded human bladder carcinoma stained with TCF4 Mouse Monoclonal Antibody (TCF4/2252) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

TCF4 (Transcription Factor 4) Antibody - Image 2

Formalin-fixed, paraffin-embedded human breast carcinoma stained with TCF4 Mouse Monoclonal Antibody (TCF4/2252) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

TCF4 Antibody in SDS-PAGE

TCF4 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Transcription factor 4 Mouse Monoclonal Antibody (TCF4/2252). Confirmation of Purity and Integrity of Antibody.

TCF4 Antibody in SDS-PAGE

TCF4 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Transcription factor 4 Mouse Monoclonal Antibody (TCF4/2252). Confirmation of Purity and Integrity of Antibody.

  • ELISA
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
ELISA, Immunohistochemistry
Species Reactivity
Human
Clone
TCF4/2252
Isotype
IgG2c
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
TCF4
Positive Control
colon or tonsil., HeLa or HepG2 cells. Human bladder
Immunogen
Recombinant human TCF4 protein fragment (around aa 365-671) (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription (By similarity). Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.
Key References
  • Van de Wetering, M., et al. 1991. Identification and cloning of TCF-1, a T lymphocyte-specific transcription factor containing a sequence-specific HMG box. EMBO J. 10: 123-132.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6925
SwissProt
P15884
Chromosome Location
18q21.2
Mol. Weight of Antigen
60kDa
Alternate Names
Transcription factor 4, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, SL3-3 enhancer factor 2, bHLHb19; Class B basic helix-loop-helix protein 19; Immunoglobulin transcription factor 2; ITF-2; PTHS; SEF2; SL3-3 enhancer factor 2; TCF4; Transcription factor 4

Summary

Recognizes a protein of 71kDa, identified as Transcription Factor 4 (TCF4). It is a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box (‘E-box’) binding site (‘CANNTG’) – a motif first identified in immunoglobulin enhancers. This gene is broadly expressed and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome.

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