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Recombinant Smooth Muscle Myosin Heavy Chain (SM-MHC)(Leiomyosarcoma & Myoepithelial Cell Marker) Antibody [MYH11/7610R]

In Stock
Catalog Number Formulation Size Price
4629-RBM10-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4629-RBM10-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4629-RBM10-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Smooth Muscle Myosin, heavy chain (SMM-HC) is a cytoplasmic structural protein that is a major component of the contractile apparatus of the smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early and is specific for smooth muscle development. SM-MHC stains the intact myoepithelial cell (MEC) layers present in benign and in situ malignant breast and bronchioloalveolar lesions and is therefore very helpful in distinguishing between benign and malignant tumors. The antibody reacts with smooth muscle cells and myoepithelial cells, but not with myofibroblasts. It is very helpful in distinguishing between benign sclerosing breast lesions and infiltrating carcinomas in difficult cases since it strongly stains the myoepithelial layer in the benign lesions while it is negative in the infiltrating carcinomas.

Product Properties & Targets

Host
Rabbit
Applications
Species Reactivity
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm.
Gene Name
Positive Control
Human smooth muscle|uterus or breast.
Immunogen
Recombinant fragment (around aa1-200) of human MYH11 protein (exact sequence is proprietary)
Alternate Names
Myosin-11, Myosin heavy chain 11, Myosin heavy chain, smooth muscle isoform, SMMHC, AAT4; Myosin heavy chain 11 (MYH11); Myosin heavy chain 11 smooth muscle; Smooth muscle myosin heavy chain 11; Myosin-11; SM1; SM2; SMHC; SMMHC

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
MYH11/7610R
Chromosome Location
16p13.11
Mol. Weight of Antigen
227kDa

Functions

  • Muscle contraction.

Key References

  • Hilson JB, et al. Am J Surg Pathol. 2010 Jun; 34(6):896-900.
  • Nicolas MM, et al. Hum Pathol. 2010 May; 41(5):663-71

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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