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SLC18A2 Antibody [SLC18A2/7983]

Additional information

Catalog Number: 6571-MSM3
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6571-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6571-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6571-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
SLC18A2 Antibody

SDS-PAGE Analysis of Purified SLC18A2 Mouse Monoclonal Antibody (SLC18A2/7983). Confirmation of Purity and Integrity of Antibody.

SLC18A2 Antibody - Image 2

Formalin-fixed, paraffin-embedded human prostate stained with SLC18A2 Mouse Monoclonal Antibody (SLC18A2/7983). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

SLC18A2 Antibody - Image 3

Formalin-fixed, paraffin-embedded human brain stained with SLC18A2 Mouse Monoclonal Antibody (SLC18A2/7983). Inset: PBS instead of primary antibody; secondary only negative control.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
SLC18A2/7983
Isotype
IgG2
Light Chain
Kappa
Cellular Localization
Cytoplasm. Vesicle membrane.
Gene Name
SLC18A2
Positive Control
adrenal gland or tonsil.|Human brain
Immunogen
Recombinant fragment of human SLC18A2 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Electrogenic antiporter that exchanges one cationic monoamine with two intravesicular protons across the membrane of secretory and synaptic vesicles. Uses the electrochemical proton gradient established by the V-type proton-pump ATPase to accumulate high concentrations of monoamines inside the vesicles prior to their release via exocytosis. Transports a variety of catecholamines such as dopamine, adrenaline and noradrenaline, histamine, and indolamines such as serotonin (PubMed:8643547, PubMed:23363473). Regulates the transvesicular monoaminergic gradient that determines the quantal size. Mediates somatodendritic dopamine release in hippocampal neurons, likely as part of a regulated secretory pathway that integrates retrograde synaptic signals (By similarity). Acts as a primary transporter for striatal dopamine loading ensuring impulse-dependent release of dopamine at the synaptic cleft (By similarity). Responsible for histamine and serotonin storage and subsequent corelease from mast cell granules (PubMed:8860238) (By similarity).
Key References
  • Henry, J.P., et al. 1994. Biochemistry and molecular biology of the vesicular monoamine transporter from chromaffin granules. J. Exp. Biol. 196: 251-262.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6571
SwissProt
Q05940
Chromosome Location
10q25.3
Mol. Weight of Antigen
63kDa
Alternate Names
Synaptic vesicular amine transporter, Monoamine transporter, Solute carrier family 18 member 2, Vesicular amine transporter 2, MNAT; Monoamine neurotransmitter transporter; Monoamine transporter; SLC18A2; Solute carrier family 18 (vesicular monoamine) member 2; SVAT; SVMT; Synaptic vesicle monoamine transporter brain; VAT2; Vesicle monoamine/H+ antiporter; Vesicular monoamine transporter 2; VMAT2

Summary

Neurotransmission depends on the regulated exocytotic release of chemical transmitter molecules. This requires the packaging of these substances into the specialized secretory vesicles of neurons and neuroendocrine cells, a process mediated by specific vesicular transporters. The family of genes encoding the vesicular transporters of monoamines (VMAT 1 and VMAT 2) and acetylcholine (VACht) have been cloned and functionally characterized. The sequence of these integral membrane proteins predicts twelve transmembrane domains and weak homology to a class of bacterial antibiotic resistance proteins. The vesicular transport of neurotransmitter molecules has been shown to be an active ATP- and proton dependent transport mechanism.

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