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Formalin-fixed, paraffin-embedded human lymph node stained with RXRB Mouse Monoclonal Antibody (PCRP-RXRB-2B6).
Immunofluorescence Analysis of PFA-fixed HeLa cells using RXRB Mouse Monoclonal Antibody (PCRP-RXRB-2B6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Immunofluorescence Analysis of PFA-fixed K562 cells using RXRB Mouse Monoclonal Antibody (PCRP-RXRB-2B6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Flow Cytometric Analysis of PFA-fixed U87 cells. RXRB Mouse Monoclonal Antibody (PCRP-RXRB-2B6) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Flow Cytometric Analysis of PFA-fixed HeLa cells. RXRB Mouse Monoclonal Antibody (PCRP-RXRB-2B6) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using RXRB Mouse Monoclonal Antibody (PCRP-RXRB-2B6). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
SDS-PAGE Analysis. Purified RXRB Mouse Monoclonal Antibody (PCRP-RXRB-2B6). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Two families of retinoid receptors, RARs and RXRs, have been identified. Retinoic acid receptors (RARs) include RAR伪, RAR尾 and RAR纬, each of which have a high affinity for all trans-retinoic acids and belong to the same class of nuclear transcription factors as thyroid hormone receptors, vitamin D3 receptor and ecdysone receptor. The ligand-binding domains of the RARs are highly conserved and RAR isoforms are expressed in distinct patterns throughout development and in the mature organism. Members of the retinoid X receptor (RXR) family, RXR伪, RXR尾 and RXR纬, are activated by 9-cis-RA, a stereo- and photo-isomer of all trans-RA that is expressed in vivo in both liver and kidney and may represent a widely used hormone. As is true for the RAR subfamily, the RXR receptors are closely related to each other both in their DNA-binding and ligand-binding domains and are encoded by separate genes at distinct chromosomal loci.
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