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Recombinant Uroplakin II (UPK2) (Urothelial Differentiation Marker) Antibody [UPK2/13669R]

Additional information

Catalog Number: 7379-RBM2
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
7379-RBM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7379-RBM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7379-RBM2-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Uroplakin II (UPK2) Antibody in Immunohistochemistry (IHC (P))

Uroplakin II (UPK2) Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human bladder cancer stained with Uroplakin II Recombinant Rabbit Monoclonal Antibody (UPK2/13669R). Inset: PBS instead of primary antibody; secondary only negative control.

Uroplakin II (UPK2) Antibody in SDS-PAGE

Uroplakin II (UPK2) Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Uroplakin II Recombinant Rabbit Monoclonal Antibody (UPK2/13669R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
UPK2/13669R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell membrane
Gene Name
UPK2
Positive Control
Urinary bladder.
Immunogen
Recombinant fragment (around aa36-51) of the human Uroplakin 2 protein (exact sequence is proprietary)
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in regulating the assembly of the AUM (By similarity).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
7379
SwissProt
O00526
Chromosome Location
11q23.3
Mol. Weight of Antigen
19.44kDa
Alternate Names
Uroplakin-2, UP2|UPII

Summary

Uroplakin II (UPII) is a 15-kDa protein comprising urothelial plaques, which fortify the urothelial barrier. It is a highly specific marker for tumors of urothelial origin, particularly urothelial carcinoma (UC). UPII messenger RNA has been detected in the tissue and blood of UC patients. Immunohistochemistry utilizing UPII antibodies, such as UPK2/13669R, exhibits high specificity and increased sensitivity for urothelial carcinoma compared to UPIII antibodies. UPII immunohistochemistry is valuable for differentiating urothelial carcinoma from other malignancies, including prostate adenocarcinoma or metastatic breast carcinoma. It has also shown promise for detecting micrometastases of bladder cancer. UPII expression is found in various UC subtypes like micro-papillary, plasmacytoid, and sarcomatoid variants.

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