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Recombinant TLE1 (Synovial Sarcoma Marker) Antibody [TLE1/2946R]

Additional information

Catalog Number: 7088-RBM4
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
7088-RBM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7088-RBM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7088-RBM4-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant TLE1 (Synovial Sarcoma Marker) Antibody

Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with TLE1 Rabbit Recombinant Monoclonal Antibody (TLE1/2946R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

TLE1 Antibody in SDS-PAGE

TLE1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Transducin-like enhancer protein 1 Rabbit Monoclonal Antibody (TLE1/2946R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
TLE1/2946R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
TLE1
Positive Control
HeLa, HepG2 or Jurkat cells. Endometrium Carcinoma or Sarcomas.
Immunogen
Recombinant fragment (around aa175-338) of the human TLE1 protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. Enhances FOXG1/BF-1- and HES1-mediated transcriptional repression (By similarity). The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
7088
SwissProt
Q04724
Chromosome Location
9q21.32
Mol. Weight of Antigen
83.2kDa
Alternate Names
Transducin-like enhancer protein 1, ESG|ESG1|GRG1|TLE-1

Summary

Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein1 (TLE1) is a protein that isencoded by theTLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.

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