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Recombinant S100A1 (Melanoma Marker) Antibody [S100A1/9244R]

Additional information

Catalog Number: 6271-RBM6
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6271-RBM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6271-RBM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6271-RBM6-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant S100A1 (Melanoma Marker) Antibody

SDS PAGE Analysis Purified S100A1 Rabbit Monoclonal Antibody (S100A1/9244R). Confirmation of Purity and Integrity of Antibody.

Recombinant S100A1 (Melanoma Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human melanoma stained with S100A1 Rabbit Monoclonal Antibody (S100A1/9244R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

S100A1 Antibody in Western Blot (WB)

S100A1 Antibody in Western Blot (WB)

Western Blot analysis of Human Skeletal muscle tissue lysate using S100A1 Recombinant Rabbit Monoclonal Antibody (S100A1/9244R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
S100A1/9244R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm, Mitochondrion, Sarcoplasmic reticulum
Gene Name
S100A1
Positive Control
Human brain or melanoma. Skeletal muscle.
Immunogen
Recombinant full-length human S100A1 protein
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed:23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed:22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed:12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed:11717446).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6271
SwissProt
P23297
Chromosome Location
1q21.3
Mol. Weight of Antigen
10.55kDa
Alternate Names
Protein S100-A1, S100|S100-alpha|S100A

Summary

S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain (i.e. in S-100A and S-100B) but not on the beta-chain of S-100 (i.e. in S-100B). This antibody can be used to localize S-100A in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, epithelial and myoepithelial cells of the breast, salivary and sweat glands. Neoplasms derived from these cells also express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein.

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