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Recombinant Prostate Specific Antigen (PSA) Antibody [rKLK3/6947]

Additional information

Catalog Number: 354-MSM17
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

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In Stock
Catalog Number Formulation Size Price
354-MSM17-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
354-MSM17-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
354-MSM17-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Prostate Specific Antigen (PSA) Antibody

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with PSA Recombinant Mouse Monoclonal Antibody (rKLK3/6947). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Recombinant Prostate Specific Antigen (PSA) Antibody - Image 2

SDS-PAGE Analysis of Purified PSA Recombinant Mouse Monoclonal Antibody (rKLK3/6947). Confirmation of Integrity and Purity of Antibody.

KLK3 Antibody in Western Blot (WB)

KLK3 Antibody in Western Blot (WB)

Western Blot Analysis of human Serum lysate using KLK3 Recombinant Mouse Monoclonal Antibody (rKLK3/6947).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rKLK3/6947
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Secreted
Gene Name
KLK3
Positive Control
PC12 cells. Normal prostate or prostate carcinoma tissues. Human Serum.
Immunogen
Recombinant fragment (around aa235-248) of the human PSA protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
354
SwissProt
P07288
Chromosome Location
19q13.33
Mol. Weight of Antigen
28.74kDa
Alternate Names
Prostate-specific antigen, APS|KLK2A1|PSA|hK3

Summary

Recognizes a single protein of 33-34kDa, identified as the prostate specific antigen (PSA). This MAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. This MAb makes an excellent pair with MAb 1A7G6B6 for PSA tests.

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