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Recombinant Prostate Specific Acid Phosphatase (PSAP) Antibody [ACPP/8409R]

Additional information

Catalog Number: 55-RBM7
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

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In Stock
Catalog Number Formulation Size Price
55-RBM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
55-RBM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
55-RBM7-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Prostate Specific Acid Phosphatase (PSAP) Antibody

SDS-PAGE Analysis of Purified PSAP Recombinant Rabbit Monoclonal Antibody (ACPP/8409R). Confirmation of Purity and Integrity of Antibody.

Recombinant Prostate Specific Acid Phosphatase (PSAP) Antibody - Image 2

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with PSAP Recombinant Rabbit Monoclonal Antibody (ACPP/8409R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
ACPP/8409R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell membrane, Cytoplasm, Cytosol, Lysosome membrane, Nucleus, Secreted
Gene Name
ACP3
Positive Control
PC12 cells. Normal prostate or prostate carcinoma tissue.
Immunogen
Recombinant full-length human PSAP protein
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins (PubMed:10506173, PubMed:15280042, PubMed:20498373, PubMed:9584846). Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma (PubMed:10506173, PubMed:15280042)., Tyrosine phosphatase that acts as a tumor suppressor of prostate cancer through dephosphorylation of ERBB2 and deactivation of MAPK-mediated signaling (PubMed:20498373). In addition to its tyrosine phosphatase activity has ecto-5'-nucleotidase activity in dorsal root ganglion (DRG) neurons. Generates adenosine from AMP which acts as a pain suppressor (By similarity)., (Microbial infection) Forms amyloid beta-sheet fibrils in semen. These fibrils, termed SEVI (semen-derived enhancer of viral infection) capture HIV virions, attach them to target cells and enhance infection (PubMed:18083097, PubMed:19451623, PubMed:19897482). SEVI amyloid fibrils are degraded by polyphenol epigallocatechin-3-gallate (EGCG), a constituent of green tea (PubMed:19451623). Target cell attachment and enhancement of HIV infection is inhibited by surfen (PubMed:19897482). Also similarly boosts XMRV (xenotropic murine leukemia virus-related virus) infection (PubMed:19403677).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
55
SwissProt
P15309
Chromosome Location
3q22.1
Mol. Weight of Antigen
44.57kDa
Alternate Names
Prostatic acid phosphatase, 5'-NT|ACP-3|ACPP|TM-PAP

Summary

Prostatic Acid Phosphatase (PSAP or PAP) is a member of the histidine acid phosphatase family. It is a non-specific phosphatase that is capable of dephosphorylating tyrosine residues as well as phospholipids under mildly acidic conditions. Prostatic specific acid phosphatase (PSAP) is involved in prostate epithelial differentiation. It is highly expressed in prostate, primary and metastatic carcinoma of the prostate. This marker may be helpful in pinpointing the site of origin in cases of metastatic carcinoma of the prostate, and is considered a more sensitive marker than PSA.

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