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PAX8 (Renal Cell Marker) Antibody [PAX8/12570]

Additional information

Catalog Number: 7849-MSM20
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
7849-MSM20-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
7849-MSM20-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7849-MSM20-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
PAX8 Antibody in Immunohistochemistry (IHC (P))

PAX8 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human thyroid stained with PAX8 Mouse Monoclonal Antibody (PAX8/12570). Inset: PBS instead of primary antibody; secondary only negative control.

PAX8 Antibody in SDS-PAGE

PAX8 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified PAX8 Mouse Monoclonal Antibody (PAX8/12570). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
PAX8/12570
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
PAX8
Positive Control
HeLa or Raji cells. Human renal cell carcinoma (RCC) or ovarian serous carcinoma.
Immunogen
Recombinant fragment (around aa50-300) of human PAX8 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
Key References
  • Tong GX,et al. Mod Pathol.2009 Sep;22(9):1218-27.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
7849
SwissProt
Q06710
Chromosome Location
2q13
Mol. Weight of Antigen
62kDa
Alternate Names
Paired box protein Pax-8, Paired box 8; Paired box gene 8; paired box homeotic gene 8; Paired box protein Pax-8; Paired domain gene 8; PAX8

Summary

Recognizes a protein of 62kDa, identified as PAX8. It is a member of the paired box (PAX) family of transcription factors. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas, and atypical thyroid adenomas. PAX-8 is expressed in the thyroid (and associated carcinomas), non-ciliated mucosal cells of the fallopian tubes, and simple ovarian inclusion cysts, but not normal ovarian surface epithelial cells. PAX-8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas. PAX-8 expression is reported in renal tubules as well as renal cell carcinoma, nephroblastoma, and seminoma. PAX-8 antibody may be used as an additional immunohistochemical marker for renal epithelial tumors.

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