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Formalin-fixed, paraffin-embedded human kidney stained with Parvalbumin Recombinant Rabbit Monoclonal Antibody (PVALB/12610R). Inset: PBS instead of primary antibody; secondary only negative control.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Parvalbumin alpha is a low molecular weight calcium-bound albumin (usually 9-11 kDa), structurally related to calmodulin and troponin C, and is a stable protein involved in calcium signaling. Parvalbumin is involved in physiological processes such as cell cycle regulation, second messenger production, muscle contraction, microtubule organization and light conduction. Parvalbumin alpha is found in rapidly contracting muscles, where its levels are highest, as well as in the brain and some endocrine tissues. In normal kidneys, parvoprotein has been shown to be confined to distal tubule cells and collecting tubule cells (intercellular cells).
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