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Recombinant OLIG2 (Marker of Glial Brain Tumors) Antibody [rOLIG2/8884]

Additional information

Catalog Number: 10215-MSM8
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

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In Stock
Catalog Number Formulation Size Price
10215-MSM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
10215-MSM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
10215-MSM8-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
OLIG2 Antibody in Western Blot (WB)

OLIG2 Antibody in Western Blot (WB)

Western Blot Analysis of Human Brain tissue lysate using OLIG2 Recombinant Mouse Monoclonal Antibody (rOLIG2/8884).

OLIG2 Antibody in SDS-PAGE

OLIG2 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Oligodendrocyte transcription factor 2 Mouse Monoclonal Antibody (rOLIG2/8884). Confirmation of Purity and Integrity of Antibody.

OLIG2 Antibody in Western Blot (WB)

OLIG2 Antibody in Western Blot (WB)

Western blot analysis of brain tissue lysates from human, guinea pig, rat, mouse, hamster and rabbit using OLIG2 Recombinant Mouse Monoclonal Antibody (rOLIG2/8884).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rOLIG2/8884
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
OLIG2
Positive Control
THP-1 cells. Human brain or astrocytoma.
Immunogen
Recombinant fragment (around aa255-267) of the human OLIG-2 protein (exact sequence is proprietary)
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Functions together with ZNF488 to promote oligodendrocyte differentiation. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
10215
SwissProt
Q13516
Chromosome Location
21q22.11
Mol. Weight of Antigen
32.38kDa
Alternate Names
Oligodendrocyte transcription factor 2, BHLHB1|OLIGO2|PRKCBP2|RACK17|bHLHe19

Summary

Olig2, a basic helix-loop-helix transcription factor, is involved in oligodendroglial specification. Olig2 expression has been reported in most glial tumors, such as oligodendrogliomas and astrocytomas. Although more than half of glioblastomas are positive for Olig2, expression is very weak in terms of both percentage of labeled cells and intensity. No Olig2 expression has been found in the non-glial tumors including neuro-epithelial tumors, ependymomas, sub-ependymomas, medulloblastomas, and non-neuroepithelial tumors, such as CNS lymphomas, meningiomas, schwannomas, atypical teratoid / rhabdoid tumor, and haemangioblastomas. Compared to the strong staining seen in glioma samples, a weak expression is observed in non-tumoral brain tissue (gliosis).

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