Printable Datasheet

Recombinant OLIG2 (Marker of Glial Brain Tumors) Antibody [rOLIG2/8884]

In Stock
Catalog Number Formulation Size Price
10215-MSM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
10215-MSM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
10215-MSM8-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
OLIG2 Antibody in Western Blot (WB)

OLIG2 Antibody in Western Blot (WB)

Western Blot Analysis of Human Brain tissue lysate using OLIG2 Recombinant Mouse Monoclonal Antibody (rOLIG2/8884).

OLIG2 Antibody in SDS-PAGE

OLIG2 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Oligodendrocyte transcription factor 2 Mouse Monoclonal Antibody (rOLIG2/8884). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Olig2, a basic helix-loop-helix transcription factor, is involved in oligodendroglial specification. Olig2 expression has been reported in most glial tumors, such as oligodendrogliomas and astrocytomas. Although more than half of glioblastomas are positive for Olig2, expression is very weak in terms of both percentage of labeled cells and intensity. No Olig2 expression has been found in the non-glial tumors including neuro-epithelial tumors, ependymomas, sub-ependymomas, medulloblastomas, and non-neuroepithelial tumors, such as CNS lymphomas, meningiomas, schwannomas, atypical teratoid / rhabdoid tumor, and haemangioblastomas. Compared to the strong staining seen in glioma samples, a weak expression is observed in non-tumoral brain tissue (gliosis).

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
OLIG2
Positive Control
THP-1 cells. Human brain or astrocytoma.
Immunogen
Recombinant fragment (around aa255-267) of the human OLIG-2 protein (exact sequence is proprietary)
Alternate Names
Oligodendrocyte transcription factor 2, BHLHB1|OLIGO2|PRKCBP2|RACK17|bHLHe19

Database Links

Entrez Gene ID
10215
SwissProt
Q13516

Additional Information

Clone
rOLIG2/8884
Chromosome Location
21q22.11
Mol. Weight of Antigen
32.38kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All OLIG2 Antibodies

Functions

  • Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Functions together with ZNF488 to promote oligodendrocyte differentiation. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK
Call Us Now: 1-866-2-NEOBIO (1-866-263-6246)
NeoBiotechnologies Logo
Company Info
Antibodies
Antibody Validation
Research Areas
Resources
Newsletter Signup Updates & Sales