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Recombinant OCT-4 (POU5F1) (Germ Cell Tumor Marker) Antibody [OCT4/6875R]

Additional information

Catalog Number: 5460-RBM5
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
5460-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
5460-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
5460-RBM5-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant OCT-4 (POU5F1) (Germ Cell Tumor Marker) Antibody

Formalin-fixed, paraffin-embedded human seminoma stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant OCT-4 (POU5F1) (Germ Cell Tumor Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human testis stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R). Inset: PBS instead of primary antibody, secondary negative control.

Recombinant OCT-4 (POU5F1) (Germ Cell Tumor Marker) Antibody - Image 3

Formalin-fixed, paraffin-embedded human seminoma stained with OCT4 Recombinant Rabbit Monoclonal Antibody (OCT4/6875R) at 2ug/ml. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
OCT4/6875R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
POU5F1
Positive Control
Raji or U-937 cells. Human seminoma or testicular carcinoma
Immunogen
Recombinant fragment (around aa1-134) of the human OCT3 protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
5460
SwissProt
Q01860
Chromosome Location
6p21.33
Mol. Weight of Antigen
38.57kDa
Alternate Names
POU domain, class 5, transcription factor 1, OCT3|OCT4|OCT4Borf1|OTF-3|OTF3|OTF4|Oct-3|Oct-4|Oct3/4

Summary

OCT4 is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells.OCT4 is a key regulator of self-renewal in embryonic stem cells; its expression is potentially correlated with tumorigenesis and can affect tumor recurrence or resistance to therapies. OCT4 is expressed in undifferentiated pluripotent cells, germ cells in ovary and testes. OCT4 Ab is useful in the identification of primary as well as metastatic germ cell tumors.

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