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Formalin-fixed, paraffin-embedded human Lung Adenocarcinoma stained with Napsin A Rabbit Recombinant Monoclonal Antibody (NAPSA/1865R).
Western Blot Analysis of Human Kidney lysate using Napsin-A Rabbit Recombinant Monoclonal Antibody (NAPSA/1865R).
Western Blot Analysis of Human Lung, Human Kidney, K-562, HEK293, and A549 lysates using Napsin A Recombinant Rabbit Monoclonal Antibody (NAPSA/1865R).
Western blot analysis of Human Kidney, Mouse Kidney, Rat Kidney, Hamster Kidney, Guinea pig Kidney, Rabbit Kidney, Human Lung, Mouse Lung, Rat Lung, Hamster Lung, Guinea pig Lung, and Rabbit Lung tissue lysates using Napsin A Recombinant Rabbit Monoclonal Antibody (NAPSA/1865R).
Western Blot Analysis of Human Brain, Human Heart, Human Intestine, Human Kidney, Human Liver, Human Lung, Human Ovary, Human Pancreas, Human Prostrate, Human Skeletal Muscle, Human Skin, Human Spleen, Human Stomach, and Human Testis tissue lysates using Napsin A Recombinant Rabbit Monoclonal Antibody (NAPSA/1865R).
SDS-PAGE Analysis of Purified Napsin-A Recombinant Rabbit Monoclonal Antibody (NAPSA/1865R). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.
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