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Recombinant MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody [MUC1/7797R]

Additional information

Catalog Number: 4582-RBM33
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4582-RBM33-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4582-RBM33-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4582-RBM33-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody

SDS-PAGE Analysis of Purified MUC1 Recombinant Rabbit Monoclonal Antibody (MUC1/7797R). Confirmation of Purity and Integrity of Antibody.

Recombinant MUC1 / CA15-3 / EMA / CD227 (Epithelial Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human ovarian cancer stained with MUC1 Recombinant Rabbit Monoclonal Antibody (MUC1/7797R). Inset: PBS instead of primary antibody; secondary only negative control.

MUC1 Antibody in Western Blot (WB)

MUC1 Antibody in Western Blot (WB)

Western Blot Analysis of Human kidney and Human Lung tissue lysates using MUC-1 Recombinant Mouse Monoclonal Antibody (MUC1/7797R).

MUC1 Antibody in Western Blot (WB)

MUC1 Antibody in Western Blot (WB)

Western Blot Analysis of Human Kidney, Mouse Kidney, Rat Kidney, Hamster Kidney, Guinea pig Kidney, Rabbit Kidney, Human Lung, Mouse Lung, Rat Lung, Hamster Lung, Guinea pig Lung and Rabbit Lung tissue lysates using MUC-1 Recombinant Mouse Monoclonal Antibody (MUC1/7797R).

  • IHC
  • WB
  • Flow
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot, Flow
Species Reactivity
Human
Clone
MUC1/7797R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Apical cell membrane, Cell membrane, Cytoplasm, Nucleus, Secreted
Gene Name
MUC1
Positive Control
MCF-7 or MDA-231 cells. Human colon, kidney, lung, breast, ovarian or endometrial carcinoma.
Immunogen
Human milk fat globule membrane
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack., The beta subunit contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4582
SwissProt
P15941
Chromosome Location
1q22
Mol. Weight of Antigen
122.1kDa
Alternate Names
Mucin-1, ADMCKD|ADMCKD1|ADTKD2|CA 15-3|CD227|Ca15-3|EMA|H23AG|KL-6|MAM6|MCD|MCKD|MCKD1|MUC-1|MUC-1/SEC|MUC-1/X|MUC1/ZD|PEM|PEMT|PUM

Summary

In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. This MAb reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. �In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

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