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Recombinant MCM6 (Proliferation Marker) Antibody [MCM6/12974R]

Additional information

Catalog Number: 4175-RBM3
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4175-RBM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4175-RBM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4175-RBM3-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
MCM6 Antibody in Western Blot (WB)

MCM6 Antibody in Western Blot (WB)

Western Blot Analysis of A-431 lysate using MCM6 Recombinant Rabbit Monoclonal Antibody (MCM6/12974R).

MCM6 Antibody in SDS-PAGE

MCM6 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified MCM6 Recombinant Rabbit Monoclonal Antibody (MCM6/12974R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
MCM6/12974R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Chromosome, Nucleus
Gene Name
MCM6
Positive Control
A-431, Human skin or squamous cell carcinoma (IHC).
Immunogen
Recombinant fragment (around aa228-368) of the human MCM6 protein (exact sequence is proprietary)
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:16899510, PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232, PubMed:9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4175
SwissProt
Q14566
Chromosome Location
2q21.3
Mol. Weight of Antigen
92.89kDa
Alternate Names
DNA replication licensing factor MCM6, MCG40308|Mis5|P105MCM

Summary

The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM6 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1/S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the post-replicative state of the cell. MCM2/MCM6 complexes function as substrates for Cdc2/cyclin B in vitro.

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