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Recombinant GP2 (Glycoprotein 2) / ZAP75 Antibody [rGP2/8617]

In Stock
Catalog Number Formulation Size Price
2813-MSM13-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2813-MSM13-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2813-MSM13-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant GP2 (Glycoprotein 2) / ZAP75 Antibody

Formalin-fixed, paraffin-embedded human pancreas stained with GP2 Recombinant Mouse Monoclonal Antibody (rGP2/8617). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant GP2 (Glycoprotein 2) / ZAP75 Antibody - Image 2

SDS-PAGE Analysis of Purified GP2 Recombinant Mouse Monoclonal Antibody (rGP2/8617). Confirmation of Integrity and Purity of the Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

GP2 (glycoprotein 2), also known as ZAP75, is a 537 amino acid secreted protein. It is an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. GP2 is cleaved and then released into the pancreatic duct along with exocrine secretions. GP2 binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). GP2 is also expressed on the apical plasma membrane of specialized microfold (M) cells among enterocytes and serves as a transcytotic receptor for mucosal antigens. M cells are considered a promising target for oral vaccination against various infectious diseases.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Apical cell membrane, Cell membrane, Endosome, Membrane raft, Secreted, Zymogen granule membrane
Gene Name
GP2
Positive Control
PANC-1 cells. Human pancreas.
Immunogen
Recombinant fragment (around aa35-179) of the human GP2 protein (exact sequence is proprietary)
Alternate Names
Pancreatic secretory granule membrane major glycoprotein GP2, ZAP75

Database Links

Entrez Gene ID
2813
SwissProt
P55259

Additional Information

Clone
rGP2/8617
Chromosome Location
16p12.3
Mol. Weight of Antigen
59.48kDa
Expression System
HEK293 Cells
Purification
Protein G

Related Antibodies

View All GP2 Antibodies

Functions

  • Functions as an intestinal M-cell transcytotic receptor specific for type-I-piliated bacteria that participates in the mucosal immune response toward these bacteria. At the apical membrane of M-cells it binds fimH, a protein of the bacteria type I pilus tip. Internalizes bound bacteria, like E.coli and S.typhimurium, from the lumen of the intestine and delivers them, through M-cells, to the underlying organized lymphoid follicles where they are captured by antigen-presenting dendritic cells to elicit a mucosal immune response.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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