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Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Galectin-1 Monospecific Recombinant Rabbit Monoclonal Antibody (GAL1/2499R).
Analysis of Protein Array containing more than 19,000 full-length human proteins using Galectin-1 Monospecific Recombinant Rabbit Monoclonal Antibody (GAL1/2499R) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
Western blot analysis of Human Brain, Human Intestine, Human Heart, Human Kidney, Human Ovary, Hela, and K-562 lysates using Galectin-1 Recombinant Rabbit Monoclonal Antibody (GAL1/2499R).
Western Blot Analysis of Human Intestine, Mouse Intestine, Rat Intestine, Hamster Intestine, Guinea Pig Intestine, Rabbit Intestine, Human Heart, Mouse Heart, Rat Heart, Hamster Heart, Guinea pig Heart, Rabbit Heart, Human Kidney, Mouse Kidney, Rat Kidney, Hamster Kidney, Guinea pig Kidney and Rabbit Kidney tissue lysates using Galectin-1 Recombinant Rabbit Monoclonal Antibody (GAL1/2499R).
SDS-PAGE Analysis of Purified Galectin-1 Recombinant Rabbit Monoclonal Antibody (GAL1/2499R). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Galectin-1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14kD participating in a variety of normal and pathological processes, including cancer progression. Galectin-1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin-1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 molecules present on the surface of lymphocytes. Galectin-1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.
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