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Recombinant FOXL2 Antibody [FOXL2/8362R]

Additional information

Catalog Number: 668-RBM3
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
668-RBM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
668-RBM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
668-RBM3-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant FOXL2 Antibody

Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with FOXL2 Recombinant Rabbit Monoclonal Antibody (FOXL2/8362R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant FOXL2 Antibody - Image 2

Formalin-fixed, paraffin-embedded human parathyroid stained with FOXL2 Recombinant Rabbit Monoclonal Antibody (FOXL2/8362R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Recombinant FOXL2 Antibody - Image 3

SDS-PAGE Analysis of Purified FOXL2 Recombinant Rabbit Monoclonal Antibody (FOXL2/8362R). Confirmation of Purity and Integrity of Antibody.

FOXL2 Antibody in Western Blot (WB)

FOXL2 Antibody in Western Blot (WB)

Western Blot Analysis of K-562 cell lysate using FOXL2 Rabbit Recombinant Monoclonal Antibody (FOXL2/8362R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
FOXL2/8362R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
FOXL2
Positive Control
Nuclear expression in ovarian follicular and stromal cells. Granulosa cell tumor. K-562.
Immunogen
Recombinant fragment (around aa247-261) of the human FOXL2 protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
668
SwissProt
P58012
Chromosome Location
3q22.3
Mol. Weight of Antigen
38.77kDa
Alternate Names
Forkhead box protein L2, BPES|BPES1|PFRK|PINTO|POF3

Summary

FOXL2 is a critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. It prevents trans-differentiation of ovary to testis through transcriptional repression of the sertoli cell-promoting gene SOX9. FOXL2 is mainly expressed in ovary, parathyroid gland and sex cord-stromal tumors. FOXL2 is generally used for diagnosis of sex cord-stromal tumors with Inhibin, Calretinin and SF-1.

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