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Recombinant Filaggrin (Keratinocyte Differentiation Marker) Antibody [rFLG/1561]

Additional information

Catalog Number: 2312-MSM6
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
2312-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2312-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2312-MSM6-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Filaggrin (Keratinocyte Differentiation Marker) Antibody

Formalin-fixed, paraffin-embedded human Skin stained with Filaggrin Mouse Recombinant Monoclonal Antibody (rFLG/1561).

Recombinant Filaggrin (Keratinocyte Differentiation Marker) Antibody - Image 2

SDS-PAGE Analysis Purified Filaggrin Mouse Recombinant Monoclonal Antibody (rFLG/1561). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rFLG/1561
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cytoplasmic granule
Gene Name
FLG
Positive Control
U-251-MG cells. Skin.
Immunogen
Recombinant fragment (around aa198-288) of the human Filaggrin (recombinant fragment-1) protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2312
SwissProt
P20930
Chromosome Location
1q21.3
Mol. Weight of Antigen
435.16kDa
Alternate Names
Filaggrin, ATOD2|FLG-1|FLG1

Summary

Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules.Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

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