Trial Size Available
Carrier-Free Available
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 18Rabbit Recombinant Monoclonal Antibody (KRT18/2819R).
Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18Rabbit Recombinant Monoclonal Antibody (KRT18/2819R).
Immunofluorescence Analysis of MCF-7 cells labeling KRT18 with Cytokeratin 18 Rabbit Recombinant MAb (KRT18/2819R followed by Goat anti-Rabbit IgG-CF488 (Cyan). The nuclear counterstain is Reddot (Red)
Flow Cytometric Analysis of PFA-fixed HeLa cells using Cytokeratin 18 Rabbit Recombinant MAb(KRT18/2819R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).
Western Blot Analysis of Human Intestine, Human Liver, Human Lung, A431, and HeLa lysates using Cytokeratin 18 Recombinant Rabbit Monoclonal Antibody (KRT18/2819R).
SDS-PAGE Analysis of Purified Keratin, type I cytoskeletal 18 Recombinant Rabbit Monoclonal Antibody (KRT18/2819R). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
0
4
4
3
6
4
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Should a NeoBiotechnologies antibody not meet the experimental expectations outlined on our website or datasheet, please contact our Technical Support. We unconditionally guarantee our product.
Our team of scientists are ready to help you with expert recommendations or technical assistance related to applications or product use.
Information
© 2026 NeoBiotechnologies. All Rights Reserved.
Monday-Friday: 8:00am – 8:00pm Pacific Time
