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Recombinant CD45RO / LCA (Activated T-cell Marker) Antibody [T200/8144R]

Additional information

Catalog Number: 5788-RBM48
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
5788-RBM48-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
5788-RBM48-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
5788-RBM48-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD45RO Antibody in Immunohistochemistry (IHC (P))

CD45RO Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human tonsil stained with CD45RO Recombinant Rabbit Monoclonal Antibody (T200/8144R). Inset: PBS instead of primary antibody; secondary only negative control.

CD45RO Antibody in Immunohistochemistry (IHC (P))

CD45RO Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human tonsil stained with CD45RO Recombinant Rabbit Monoclonal Antibody (T200/8144R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

CD45RA / LCA Antibody in Western Blot (WB)

CD45RA / LCA Antibody in Western Blot (WB)

Western blot analysis of MOLT4 cell lysate using CD45RO Rabbit Recombinant Monoclonal Antibody (T200/8144R).

PTPRC Antibody in SDS-PAGE

PTPRC Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD45RO Recombinant Rabbit Monoclonal Antibody (T200/8144R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
T200/8144R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell membrane, Cell surface
Gene Name
PTPRC
Positive Control
Human tonsil or lymph node. MOLT4 cells.
Immunogen
Recombinant full-length human PTPRC protein
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
  • (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
Key References
  • Gatter KC et. al. Lancet, 1985 Jun 8, 1(8441):1302-5.
  • Michie SA et. al. American Journal of Clinical Pathology, 1987, 88(4):457-62.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
5788
SwissProt
P08575-4
Chromosome Location
1q31.3
Mol. Weight of Antigen
180-220kDa
Alternate Names
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Summary

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

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