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Recombinant CD45RO / LCA (Activated T-cell Marker) Antibody [T200/8144R]

In Stock
Catalog Number Formulation Size Price
5788-RBM48-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
5788-RBM48-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
5788-RBM48-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD45RO Antibody in Immunohistochemistry (IHC (P))

CD45RO Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human tonsil stained with CD45RO Recombinant Rabbit Monoclonal Antibody (T200/8144R). Inset: PBS instead of primary antibody; secondary only negative control.

CD45RO Antibody in Immunohistochemistry (IHC (P))

CD45RO Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human tonsil stained with CD45RO Recombinant Rabbit Monoclonal Antibody (T200/8144R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

CD45RA / LCA Antibody in Western Blot (WB)

CD45RA / LCA Antibody in Western Blot (WB)

Western blot analysis of MOLT4 cell lysate using CD45RO Rabbit Recombinant Monoclonal Antibody (T200/8144R).

PTPRC Antibody in SDS-PAGE

PTPRC Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD45RO Recombinant Rabbit Monoclonal Antibody (T200/8144R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Developmental Biology, Immunology, B Cell Markers, Dendritic Cell Marker, Hematopoietic Stem Cells, Mesenchymal Stem Cell Differentiation
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Cell surface
Gene Name
PTPRC
Positive Control
Human tonsil or lymph node. MOLT4 cells.
Immunogen
Recombinant full-length human PTPRC protein
Alternate Names
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

Database Links

Entrez Gene ID
5788
SwissProt
P08575-4

Additional Information

Clone
T200/8144R
Chromosome Location
1q31.3
Mol. Weight of Antigen
180-220kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All PTPRC Antibodies

Functions

  • Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
  • (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

Key References

  • Gatter KC et. al. Lancet, 1985 Jun 8, 1(8441):1302-5.
  • Michie SA et. al. American Journal of Clinical Pathology, 1987, 88(4):457-62.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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