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Recombinant CD4 (T-Helper/Inducer Cell Marker) Antibody [rCD4/3930]

Additional information

Catalog Number: 920-MSM10
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

download data sheet
In Stock
Catalog Number Formulation Size Price
920-MSM10-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
920-MSM10-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
920-MSM10-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD4 (T-Helper/Inducer Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human lymph node stained with CD4 Recombinant Mouse Monoclonal Antibody (rCD4/3930). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Recombinant CD4 (T-Helper/Inducer Cell Marker) Antibody - Image 2

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using rCD4/3930 at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Recombinant CD4 (T-Helper/Inducer Cell Marker) Antibody - Image 3

Formalin-fixed, paraffin-embedded human tonsil stained with CD4 Recombinant Mouse Monoclonal Antibody (rCD4/3930).

Recombinant CD4 (T-Helper/Inducer Cell Marker) Antibody - Image 4

SDS-PAGE Analysis Purified CD4 Recombinant Mouse Monoclonal Antibody (rCD4/3930). Confirmation of Integrity and Purity of Antibody.

Recombinant CD4 (T-Helper/Inducer Cell Marker) Antibody - Image 5

Formalin-fixed, paraffin-embedded human lymph node stained with CD4 Recombinant Mouse Monoclonal Antibody (rCD4/3930).

CD4 Antibody in Western Blot (WB)

CD4 Antibody in Western Blot (WB)

Western Blot Analysis of human tonsil tissue lysate using CD4 Recombinant Mouse Monoclonal Antibody (rCD4/3930).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rCD4/3930
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cell membrane
Gene Name
CD4
Positive Control
CCRF-CEM or HL-60. Human lymph node or tonsil., Human peripheral blood mononuclear cells (PBMCs)
Immunogen
Recombinant fragment (around aa216-396) of the human CD4 protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages., (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed:12089508, PubMed:16331979, PubMed:2214026, PubMed:9641677). Down-regulated by HIV-1 Vpu (PubMed:17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed:7909607).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
920
SwissProt
P01730
Chromosome Location
12p13.31
Mol. Weight of Antigen
51.11kDa
Alternate Names
T-cell surface glycoprotein CD4, CD4mut|IMD79|Leu-3|OKT4D|T4

Summary

Recognizes a protein of 55kDa, identified as CD4. It is a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. The majority of peripheral T-cell lymphomas are derived from the T-helper/regulatory cell subset so that most mature T-cell neoplasms are CD4+/CD8-. Anti-CD4 is used in the immunohistochemical staining of lymphoproliferative disorders to evaluate tumors with CD4 aberrant expression.

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