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Recombinant CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Antibody [r137B4]

In Stock
Catalog Number Formulation Size Price
939-MSM25-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
939-MSM25-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
939-MSM25-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD27 Antibody in Immunohistochemistry (IHC (P))

CD27 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human tonsil stained with CD27 Recombinant Mouse Monoclonal Antibody (r137B4). Inset: PBS instead of primary antibody; secondary only negative control.

CD27 Antibody in SDS-PAGE

CD27 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD27 Recombinant Mouse Monoclonal Antibody (r137B4). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

CD27 (also known as S152 or T14) is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell costimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFκB and SAPK/JNK and induces apoptosis.

Product Properties & Targets

Antibody Type
Mouse Monoclonals, Recombinant Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Immuno Oncology, Immunology, AKT Signaling, B Cell Markers, Cytokine Signaling, Infectious Disease
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane
Gene Name
CD27
Positive Control
Ramos cells. Human peripheral blood lymphocytes. Human tonsil and stomach tissues.
Immunogen
Recombinant human CD27 protein
Alternate Names
CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, LPFS2; S152; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFRSF7; TNFSF7; Tp55; Tumor necrosis factor receptor superfamily member 7

Database Links

Entrez Gene ID
939
SwissProt
P26842

Additional Information

Clone
r137B4
Chromosome Location
12p13
Mol. Weight of Antigen
120kDa

Related Antibodies

View All CD27 Antibodies

Functions

  • Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells (PubMed:28011863). The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells (PubMed:28011863). Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory (PubMed:38354704). In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis (By similarity). Negatively regulates the function of Th2 lymphocytes in the adipose tissue (By similarity).

Key References

  • De Rie MA, et al. Clin Exp Dermatol.1996 Mar;21(2):104-11.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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