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Recombinant CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Antibody [rLPFS2/1611]

In Stock
Catalog Number Formulation Size Price
939-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
939-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
939-MSM3-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with CD27 Recombinant Mouse Monoclonal Antibody (rLPFS2/1611).

Recombinant CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Antibody - Image 2

SDS-PAGE Analysis of Purified CD27 Recombinant Mouse Monoclonal Antibody (rLPFS2/1611). Confirmation of Integrity and Purity of Antibody.

Recombinant CD27 (Tumor Necrosis Factor Receptor Superfamily 7) Antibody - Image 3

Flow Cytometric Analysis of Ramos cells using CD27 Recombinant Mouse Monoclonal Antibody (rLPFS2/1611) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFkB and SAPK/JNK and induces apoptosis.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, Flow
Species Reactivity
Human
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell membrane
Gene Name
CD27
Positive Control
Ramos cells. Human peripheral blood lymphocytes. Human tonsil and lymph node.
Immunogen
Recombinant human CD22 protein (exact sequence is proprietary)
Alternate Names
CD27 antigen, S152|S152. LPFS2|T14|TNFRSF7|Tp55

Database Links

Entrez Gene ID
939
SwissProt
P26842

Additional Information

Clone
rLPFS2/1611
Chromosome Location
12p13.31
Mol. Weight of Antigen
29.14kDa
Expression System
HEK293 Cells
Purification
Protein G

Related Antibodies

View All CD27 Antibodies

Functions

  • Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells (PubMed:28011863). The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells (PubMed:28011863). Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory (PubMed:38354704). In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis (By similarity). Negatively regulates the function of Th2 lymphocytes in the adipose tissue (By similarity).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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