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Recombinant CD171 / NCAM-L1 (Axonal Marker) Antibody [rL1CAM/9163]

In Stock
Catalog Number Formulation Size Price
3897-MSM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3897-MSM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3897-MSM8-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
L1CAM Antibody in SDS-PAGE

L1CAM Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Neural cell adhesion molecule L1 Recombinant Mouse Monoclonal Antibody (rL1CAM/9163). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

L1 (L1 cell adhesion molecule) also known as CAML1, CD171, and HSAS is an adhesion molecule which plays an important role in the nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). This mouse monoclonal antibody recognizes the sequence immediately NH2-terminal to the tyrosine-based motif and binds L1 only when Y1176 is dephosphorylated. Phosphorylation of either T1172 or Y1176 strongly inhibits binding of 74-5H7 to the L1 cytoplasmic domain.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot, Flow
Species Reactivity
Human
Isotype / Light Chain
IgG2b / Kappa
Cellular Localization
Axon, Cell membrane, Cell projection, Dendrite, Growth cone
Gene Name
L1CAM
Positive Control
Human brain.
Immunogen
Recombinant fragment (around aa1-1257) of the human CD171 protein (exact sequence is proprietary)
Alternate Names
Neural cell adhesion molecule L1, CAML1|CD171|HSAS|HSAS1|HYCX|MASA|MIC5|N-CAM-L1|N-CAML1|NCAM-L1|S10|SPG1

Database Links

Entrez Gene ID
3897
SwissProt
P32004

Additional Information

Clone
rL1CAM/9163
Chromosome Location
Xq28
Mol. Weight of Antigen
140.0kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All L1CAM Antibodies

Functions

  • Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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