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Recombinant CD14 (Monocyte / Macrophage Marker) Antibody [LPSR/13729R]

Additional information

Catalog Number: 929-RBM28
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

download data sheet
CD14 Antibody in Western Blot (WB)

CD14 Antibody in Western Blot (WB)

Western Blot Analysis of Human Liver tissue lysates using CD14 Recombinant Rabbit Monoclonal Antibody (LPSR/13729R).

  • IHC
  • WB
In Stock
Catalog Number Formulation Availability Size Price
929-RBM28-P0
Purified Ab with BSA and Azide at 200ug/ml
Trial Size
In Stock
20ug
$259.00
Trial Size Available In-Stock
929-RBM28-P1
Purified Ab with BSA and Azide at 200ug/ml
In Stock
100ug
$559.00
In-Stock
929-RBM28-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
Carrier-Free
In Stock
100ug
$559.00
Carrier-Free Available In-Stock
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
LPSR/13729R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell membrane, Golgi apparatus, Membrane raft, Secreted
Gene Name
CD14
Positive Control
Human lymph node or tonsil.
Immunogen
Recombinant fragment (around aa1-100) of the human CD14 protein (exact sequence is proprietary)
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Additional information

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:22265692, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
929
SwissProt
P08571
Chromosome Location
5q31.3
Mol. Weight of Antigen
40.08kDa
Alternate Names
Monocyte differentiation antigen CD14, -

Summary

CD14 is receptor for endotoxin and apoptotic cells. It is expressed by monocytes/histiocytes, Langerhans’ cells, follicular dendritic reticulum cells, neutrophils (weakly), and endothelial cells of the liver sinusoids.CD14 is detected in myeloid leukemias with monoblastic/monocytic differentiation, and in histiocytic neoplasms like histiocytic lymphoma, Langerhans’ cell histiocytosis and giant cell tumor. In cultures, a subpopulation of Kaposi sarcoma cells express CD14. In differential diagnosis, CD14 is more specific than CD68 in the identification of monocytes/histiocytes and associated neoplasias.

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