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Recombinant CD14 (Monocyte / Macrophage Marker) Antibody [rLPSR/2408]

Additional information

Catalog Number: 929-MSM12
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
929-MSM12-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
929-MSM12-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
929-MSM12-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD14 (Monocyte / Macrophage Marker) Antibody

Formalin-fixed, paraffin-embedded human spleen stained with CD14 Recombinant Mouse Monoclonal Antibody (rLPSR/2408).

Recombinant CD14 (Monocyte / Macrophage Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human spleen stained with CD14 Recombinant Mouse Monoclonal Antibody (rLPSR/2408).

Recombinant CD14 (Monocyte / Macrophage Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified CD14 Recombinant Mouse Monoclonal Antibody (rLPSR/2408). Confirmation of Purity and Integrity of Antibody.

CD14 Antibody in Western Blot (WB)

CD14 Antibody in Western Blot (WB)

Western Blot Analysis of Human Kidney and Human Liver tissue lysates using CD14 Recombinant Mouse Monoclonal Antibody (rLPSR/2408).

CD14 Antibody in Western Blot (WB)

CD14 Antibody in Western Blot (WB)

Western Blot Analysis of Liver tissue lysates of different species using CD14 Recombinant Mouse Monoclonal Antibody (rLPSR/2408).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rLPSR/2408
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell membrane, Golgi apparatus, Membrane raft, Secreted
Gene Name
CD14
Positive Control
A549, THP-1 cells and human monocytes. Lymph nodes and tonsils. Kidney, Liver.
Immunogen
Recombinant fragment (around aa4-124) of the human CD14 protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:22265692, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
929
SwissProt
P08571
Chromosome Location
5q31.3
Mol. Weight of Antigen
40.08kDa
Alternate Names
Monocyte differentiation antigen CD14, -

Summary

Recognizes a protein of 55kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.

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