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Carrier-Free Available
Formalin-fixed, paraffin-embedded human spleen stained with Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/2210R).
Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/2210R).
Formalin-fixed, paraffin-embedded human Follicular Lymphoma stained with Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/2210R).
Analysis of Protein Array containing more than 19,000 full-length human proteins using Bcl-2 Rabbit Recombinant Monoclonal Antibody (BCL2/2210R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western blot analysis of Human Spleen, Human Skeletal Muscle, and THP-1 lysates using BCL2 Recombinant Rabbit Monoclonal Antibody (BCL2/2210R).
Western blot analysis of Human Spleen, Mouse Spleen, Rat Spleen, Hamster Spleen, Guinea pig Spleen, Rabbit Spleen, Human Skeletal Muscle, Mouse Skeletal Muscle, Rat Skeletal Muscle, Hamster Skeletal Muscle, Guinea pig Skeletal Muscle and Rabbit Skeletal Muscle tissue lysates using BCL2 Recombinant Rabbit Monoclonal Antibody (BCL2/2210R).
SDS-PAGE Analysis of Purified Apoptosis regulator Bcl-2 Recombinant Rabbit Monoclonal Antibody (BCL2/2210R). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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This antibody recognizes a protein of 25-26kDa, identified as the bcl-2 lpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2 lpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 lpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
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