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Recombinant Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Antibody [MSVA-350R]

In Stock
Catalog Number Formulation Size Price
250-RBM13-P0
Purified Ab with BSA and Azide
20ug
$324.50
250-RBM13-P1
Purified Ab with BSA and Azide
100ug
$654.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
ALPP Antibody in Numerous Tissues (IHC)

ALPP Antibody in Numerous Tissues (IHC)

Alkaline phosphatase, placental type Rabbit Recombinant Monoclonal Antibody (MSVA-350R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Mesenchymal Stem Cell Differentiation, Stem Cell Differentiation
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane
Gene Name
ALPP
Positive Control
Placenta (mature): A strong membranous and cytoplasmic staining of cytotrophoblast and syncytiotrophoblast must be seen.
Immunogen
Recombinant full-length human ALPP protein
Alternate Names
Alkaline phosphatase, placental type, Alkaline phosphatase Regan isozyme, Placental alkaline phosphatase 1, Alkaline phosphatase placental type; Alkaline phosphatase Regan Isozyme; ALP; Alp1; ALPP; Germ-cell alkaline phosphatase; nagao Isozyme; PALP; Placental alkaline phosphatase 1; placental heat-stable alkaline phosphatase; PLAP-1; PLAP1

Database Links

Entrez Gene ID
250
SwissProt
P05187

Additional Information

Clone
MSVA-350R
Chromosome Location
2q37.1
Mol. Weight of Antigen
70kDa

Related Antibodies

View All ALPP Antibodies

Functions

  • Alkaline phosphatase that can hydrolyze various phosphate compounds.

Key References

  • Wick, MR, et al. 1987;Hum Pathol. 18(9):946-54.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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