Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Mitochondria Mouse Monoclonal Antibody (113-1).

Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Mouse Monoclonal Antibody (113-1).

Western Blot analysis of HeLa cell lysate using Mitochondria Mouse Monoclonal Antibody (113-1).

Confocal immunofluorescence image of HeLa cells using Mitochondria Mouse Monoclonal Antibody (113-1) labeled is Green (CF488) and Reddot is used to label the nuclei Red.

SDS-PAGE Analysis Purified Mitochondria Mouse Monoclonal Antibody (113-1). Confirmation of Integrity and Purity of Antibody.

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Mitochondria Mouse Monoclonal Antibody (113-1).

Mitochondria (Marker for Human Cells, Granular RCC s & Salivary Tumor [113-1]

Name: Mitochondria (Marker for Human Cells, Granular RCC s &amp; Salivary Tumor
SKU: MSM1-739
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial MAb has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Reportedly, this MAb facilitates the classification of salivary tumors.

Printable

Product Properties & Targets

Host

Mouse

Species Reactivity

Human

Isotype

IgG1

Cellular Localization

N/A

Gene Name

N/A

Mol. Weight of Antigen

60kDa

Immunogen

Semi-Purified mitochondrial preparation

Database Links

Entrez Gene ID

Not Known

Unigene

Not Known

SwissProt

N/A

Additional Information

Clone

113-1

Human Gene Symbol

Not Known

Chromosome Location

Not Known

Known Applications & Suggested Dilutions

Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

Tickoo SK, Amin MB, Linden MD, Lee MW, Zarbo RJ. Antimitochondrial antibody (113-1) in the differential diagnosis of granular renal cell tumors. Am J Surg Pathol. 1997;21(8):922-30.
Vera-Sempere F, Vera-Sirera B. Usefulness of antimitochondrial antibody 113-1 in diagnosis and classification of salivary gland tumours with oncocytic differentiation. Acta Otorrinolaringol Esp. 2011;62(1):1-9.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.