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Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody [A103 + T311 + HMB45]

Additional information

Catalog Number: MSM2-702
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
MSM2-702-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
MSM2-702-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
MSM2-702-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody

Formalin-fixed, paraffin-embedded human Melanoma stained with Melanoma Marker Monoclonal Antibody (A103 + T311 + HMB45).

Melanoma Marker (MART-1 + Tyrosinase + gp100) Antibody - Image 2

Western Blot Analysis of COLO-38 cell lysate using Melanoma Marker MAb (A103 + T311 + HMB45).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
A103 + T311 + HMB45
Isotype
IgG1, IgG2a
Light Chain
Kappa
Cellular Localization
Endoplasmic reticulum membrane, Golgi apparatus, Melanosome, trans-Golgi network membrane
Gene Name
MLANA
Positive Control
COLO-38 or SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.
Immunogen
Recombinant hMART-1 protein (A103); Recombinant tyrosinase protein (T311); Extract of pigmented melanoma metastases from lymph nodes (HMB45)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Key References
  • Chen Y-T, et. al. Proc. Natl. Acad. Sci. USA, 1995, 92:8125-8129.
  • Chen Y-T, et. al. Proc Natl Acad Sci, USA, 1996, 93:5915-19.
  • Gown AM, et. al. American Journal of Pathology, 1986, 123(2):195-203.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2315; 7299; 6490
SwissProt
Q16655; P14679; P40967
Mol. Weight of Antigen
20-22kDa (doublet) (MART); 70-80kDa (Tyrosinase); 26-100kDa (gp100)
Alternate Names
Melanoma antigen recognized by T-cells 1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A, Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A; TYR; PMEL17

Summary

This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. The function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

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